- Minimize bias introduced by microbial growth and DNA degradation
- Eliminate the costs associated with temperature controlled shipping
- Eliminate need for weighing and aliquot bias with a standard volumetric sample
- Maintain DNA integrity in typical ambient temperature fluctuations (e.g., -20°C to 50°C)
- Minimize noise in your data analysis with reliable microbiota profile
- FOR ANIMAL USE ONLY.
- FOR EXTERNAL USE ONLY.
- Do NOT remove the yellow tube top from the tube.
- Do NOT spill the stabilizing liquid in the tube.
- Wash with water if liquid comes in contact with eyes or skin. Do NOT ingest.
- If fecal sample is liquid or animal has diarrhea, wait until the next normal bowel movement to collect.
- Small items may pose a choking hazard.
- Larger 'mail to' mailing envelope (9" x 12")
- Smaller 'return' bubble envelope marked "exempt animal specimen" (6" x 10")
- Blank mailing labels (2 per mailing solution)
- Sample Mailing Instructions (1 per mailing solution)
- MO-B kit assembly instructions
- Absorbent material in liquid-tight bag sufficient to soak up at least 4mL of liquid.
PERFORMAbiome•GUT | PB-200
Collect and stabilize DNA for quantitative animal gut microbiome profile analysis
PERFORMAbiome•GUT is a simple all-in-one system for easy collection and stabilization of DNA for animal microbiome profile analysis.
Benefits of PERFORMAbiome•GUT
Collection attributes table
Attribute
|
PERFORMAbiome•GUT (PB-200)
|
Sample homogenization
|
✔
|
Sample collected per kit (median)
|
630 ± 120 mg
|
Shipping at ambient temperature
|
✔
|
Compatible with guanidinium based extraction kits
|
✔
|
Standardized format for high-throughput processing
|
✔
|
Number of extractions per kit (250 μL per extraction)
|
8
|
DNA yield per extraction (median)
|
1.66 ± 0.8 μg
|
High molecular weight DNA
|
✔
|
Microbiome profile stability at room temperature
|
60 days
|
Suitable for NGS downstream application
|
✔
|
Quantitative analysis were performed using canine fecal samples
|
✔
|
PERFORMAbiome•GUT | PB-200
Collection
Collection precautions
Summary and explanation of the kit: PERFORMAbiome™•GUT provides the materials and instructions for collecting and stabilizing microbial DNA from an animal fecal sample.
Preparation: Have toilet paper or tissues available for the collection.
Warnings and precautions:
While holding the yellow tube top, unscrew ONLY the purple cap from the kit and set aside for later use.
IMPORTANT:
Do NOT remove the yellow tube top.
Do NOT spill the stabilizing liquid in the tube.
Use the spatula to collect a small amount of fecal sample.
Transfer the fecal sample into the yellow tube top. Repeat until the sample fills the yellow tube top.
IMPORTANT:
Do NOT push sample into the tube.
Scrape horizontally across the tube top to level the sample and remove any excess.
Wipe exterior of tube and top with tissue as needed.
Pick up the purple cap with the solid end facing down and screw onto the yellow tube top until tightly closed.
Shake the sealed tube as hard and fast as possible in a back and forth motion for a minimum of 30 seconds.
The fecal sample will be mixed with the stabilizing liquid in the tube; not all particles will dissolve.
IMPORTANT:
Continue shaking if large particles remain as shown in Figure A.
Place spatula in original packaging and discard in garbage.
IMPORTANT:
Send the sample for processing following the delivery instructions supplied separately by the kit provider.
Print instructions
PERFORMAbiome•GUT
PB-200
Data Sheets
Protocols
Safety Data Sheets
External Application Notes
Customization
Custom Packaging
Custom Packaging
The need to collect DNA samples from remote areas requires mailing logistics that can be complicated and costly. To facilitate this process, DNA Genotek has conducted research to create a mailing solution that complies with International Air Transport Association (IATA) guidelines, while also protecting the collection kit on the way to and from the donor. The solution adheres to IATA regulations specifically for animal specimens for which there is a minimal likelihood that pathogens are present.
Since courier services designated as "ground" may involve an air transport segment, the IATA publications are broadly applicable to both air and ground shipments. To ensure full adherence, we recommend that customers check for any variation based on their local postal service.
Two-way Mailer
For commercial applications the two-way mailer is ideal for situations that require a collection kit to be mailed to the customer and return shipped in the same package. The two-way mailer has sufficient space to include additional paperwork if required. Custom return label options are available. Minimal quantities apply.
Outer two-way mailer can be customized up to four colour graphics.
Inside flaps can be customized with instructions.
Inside contains one standard PG-100 DNA collection kit plus one MO-3.
Shipping Recommendations
Click here to view a guideline for shipping samples collected with the PERFORMAbiome•GUT collection kit.
Mailing Options
Mailing Options
The need to collect DNA samples from remote areas requires mailing logistics that can be complicated and costly. To facilitate this process, DNA Genotek has conducted research to create a mailing solution that complies with International Air Transport Association (IATA) guidelines, while also protecting the collection kit on the way to and from the donor. The solution adheres to IATA regulations specifically for animal specimens for which there is a minimal likelihood that pathogens are present.
Since courier services designated as "ground" may involve an air transport segment, the IATA publications are broadly applicable to both air and ground shipments. To ensure full adherence, we recommend that customers check for any variation based on their local postal service.
MO-B
MO-B provides a complete solution for your mailing studies:
Envelopes and labels: Catalogue number MO-B
MO-5
MO-5 is a liquid tight bag containing absorbent material to prevent leakage during shipping. The instructions for use and biohazard logo are printed on the bag for easy reference. The narrow shape makes it an ideal solution for transporting tubes.
Shipping Recommendations
Click here to view a guideline for shipping samples collected with the PERFORMAbiome•GUT collection kit.
The PERFORMAbiome•GUT collection devices come with straightforward directions, are convenient to use, provide a sample size sufficient for several DNA extractions, and will be extremely useful for those who need to preserve samples collected in the field.
Kelly Scott Swanson, Ph.D.
The Kraft Heinz Company Endowed Professor in Human Nutrition
Professor, Department of Animal Sciences and Division of Nutritional Sciences
Collect and stabilize high quality DNA samples for animal microbiome profiling
Full terms and conditions for all DNA Genotek products are available here.
DNA Genotek's sample collection devices and nucleic acid stabilization chemistries are protected by issued and pending patents in numerous countries around the world.