At DNA Genotek, we are proud of how our customers successfully use our products to achieve breakthroughs in genomic research. We would like to share their success with you and invite you to browse this section of our website to find out more about our enabling technology.
Bocklandt S et al. Epigenetic predictor of age. PLoS One. 6(6):e14821 (2011).
This is a very exciting paper that highlights the use of Oragene in epigenetic studies. The authors collected saliva samples from 34 pairs of identical twins and quantified the methylation status of specific genes using the Illumina HumanMethylation27 microarray. They found that the degree of cytosine methylation in these genes is significantly correlated with age.
Wessel J et al. Resequencing of Nicotinic Acetylcholine Receptor Genes and Association of Common and Rare Variants with the Fagerström Test for Nicotine Dependence. Neuropsychopharmacology. 35(12):2392-402 (2010).
Using next-generation sequencing, these researchers sequenced the exons of 10 genes in 448 European-American participants of a smoking cessation trail. DNA was extracted from saliva samples collected using Oragene self-collection kits as part of the Comprehensive Medication Program and Support Services (COMPASS) study, a randomized trial sponsored by the National Cancer Institute. The 454 Genome Sequencer System from Roche was used for the sequencing, supplemented by the Sanger sequencing method for two genes.
Koni AC et al. DNA Yield and Quality of Saliva Samples and Suitability for Large-scale Epidemiological Studies in Children. Int J Obes. 35:5113-5118 (2011).
This study is a great example of how Oragene can be used in very large, integrated, multicentre projects to increase response rates with children due to the ease of use and noninvasive collection method. The researchers used the Oragene self-collection saliva kits (OG-300 and OG-250 with the CS-1 sponge accessory) to collect high quality DNA from 14,019 children aged 2-9 years. The sponge accessories were used with children too young or unable to spit.
Patel RS et al. High Resolution of MicroRNA Signatures in Human Whole Saliva. Arch Oral Biol. Epub ahead of print (2011).
This is a great example of how both mRNA and microRNA (miRNA) can be successfully isolated from saliva samples. The researchers isolated high quantities of quality miRNA from saliva without preamplification, using the Oragene•RNA self-collection kits. The unique Oragene•RNA preservative solution allowed the samples to be stored at room temperature before the total RNA was collected and analyzed.
Yokoyama JS, Erdman CA and Hamilton SP. Array-Based Whole-Genome Survey of Dog Saliva DNA Yields High Quality SNP Data. PLoS ONE 5(5): e10809 (2010).
This publication highlights the collection of saliva-based DNA from companion animals; specifically dogs. The authors successfully obtained high concentrations of canine DNA from a quick oral sample using DNA Genotek's Oragene•ANIMAL collection kits (OA-400) for use on a genome-wide genotyping array (GWAS). The high quality DNA samples performed equally well to blood-extracted DNA when genotyped using the Illumina Infinium canine genotyping platform, with average call-rates greater than 99%!
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