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  • Products → Reagent → HEMAgene•Buffy Coat | HG-BCD

    Reagents for the stabilization of DNA

    Stabilize superior samples for your genetic analysis and banking

    HEMAgene•Buffy Coat | HG-BCD

    Stabilize high molecular weight DNA in buffy coat samples at ambient temperature

    HEMAgene®•BUFFY COAT is a DNA stabilizing reagent designed for ambient temperature transport and room temperature archival storage, while enabling flexible sample management workflows. Maximize the utility of fresh and archived buffy coat samples for downstream DNA applications.

    Overview

    Product Benefits

    • DNA from buffy coat is stable for long-term storage at room temperature
    • Simplify shipping logistics and decrease costs with elimination of cold chain
    • Eliminate risk of sample loss and degradation and reduce carbon footprint
    • Optimized for use with standard blood extraction solutions
    • Enable flexible aliquot schemes
    • Scalable solution for automated processing
    • DNA suitable for any downstream application

    Transport:

    • Eliminate cold chain for transport of buffy coat samples to enable sample sharing between multiple locations
    • Reduce packaging and shipping costs by 78% with the elimination of dry ice

    Flexibility:

    • Defer cost of DNA extraction until samples are accessed for processing
    • Compatible with freezing and can withstand multiple freeze-thaw cycles

    Storage:

    • Shield samples from degradation and eliminate risk of relocating specimens due to power failures
    • Reduce freezer footprint and temperature monitoring devices for eligible specimens

    Liquid vs. dry state stabilizer:

    • Sample remains in liquid state
    • 100% of the DNA is available for recovery
    • Unlimited application volume
    • No drying time, speed vac or rehydration required

    Literature

    Data Sheets

    Download PD-BR-00138 – HEMAgene®•BUFFY COAT data sheet.

    Protocols

    Protocols

    PD-HB-00003: HEMAgene®•ŸBUFFY COAT protocol handbook (pdf)

    White Papers

    White Papers

    PD-WP-00036: Long-term stability of DNA from buffy coat samples stored in HEMAgene®•BUFFY COAT DNA stabilizing reagent (pdf)

    PD-WP-00034: Cost savings of shipping buffy coat samples at ambient temperature using HEMAgene®•BUFFY COAT DNA stabilizing reagent (pdf)

    PD-WP-00033: HEMAgene®•BUFFY COAT DNA stabilizing reagent protects DNA in buffy coat samples through multiple freeze-thaw cycles (pdf)

    Application Notes

    Application Notes

    HEMAgene®•BUFFY COAT (HG-BCD) Preservative Compatibility with the Maxwell® 16 LEV Blood DNA Kit Application Note (pdf)

    HEMAgene®•BUFFY COAT (HG-BCD) Preservative Compatibility with ReliaPrep™ Large Volume HT DNA Isolation System Application Note (pdf)

    HEMAgene®•BUFFY COAT (HG-BCD) Preservative Compatibility with ReliaPrep™ Blood gDNA Miniprep System Application Note (pdf)

    MK-AN-00030: Automated extraction of gDNA from buffy coat samples preserved with HEMAgene®•BUFFY COAT DNA stabilizing reagent using the QIAGEN® QIAsymphony® SP (pdf)

    MK-AN-00028: Compatibility of the Agencourt® GenFind™ v2 blood and serum genomic DNA isolation kit with fresh buffy coat samples in HEMAgene®•BUFFY COAT DNA stabilizing reagent (pdf)

    MK-AN-00027: Compatibility of the Promega ReliaPrep™ Blood gDNA Miniprep System with fresh buffy coat samples in HEMAgene®•BUFFY COAT DNA stabilizing reagent (pdf)

    MK-AN-00026: Compatibility of the QIAGEN® QIAamp® DNA blood mini kit with fresh buffy coat samples in HEMAgene®•BUFFY COAT DNA stabilizing reagent (pdf)

    HEMAgene®•BUFFY COAT is for research use only, not for use in diagnostic procedures.

    Full terms and conditions for all DNA Genotek products are available here.

    DNA Genotek's sample collection devices and nucleic acid stabilization chemistries are protected by issued and pending patents in numerous countries around the world.

    Powerful reasons to switch to ambient stabilization

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    DNA Genotek Inc., a subsidiary of OraSure Technologies, Inc. (NASDAQ: OSUR), focuses on providing high-quality biological sample collection products and end-to-end services for human genomics, microbiome and infectious disease applications. The Company's Oragene•Dx and ORAcollect•Dx product lines are the first and only FDA 510(k) cleared saliva-based DNA collection devices for in vitro diagnostic use. DNA Genotek also offers Research Use Only products to collect and preserve large amounts of DNA or RNA from multiple sample types. DNA Genotek markets its products worldwide and has a global customer base with thousands of customers in over 100 countries.

    © 2022 DNA Genotek Inc., a subsidiary of OraSure Technologies, Inc., all rights reserved.

  • Industry Solutions
    • Human Genomics - Academic Research
    • Human Genomics - Clinical Diagnostics
    • Microbiome - Academic Research
    • Clinical Microbiome
    • Infectious Disease
    • Metabolomics
    • Biobanking
    • Animal
    • H L A Typing
  • Products
    • Human Genomics
      • All research DNA & RNA collection kits (saliva)
      • DNA Extraction Reagents
        • prepIT•L2P | PT-L2P (saliva)
        • prepIT•Q2A | PT-Q2A (saliva)
        • HEMAgene | HG-BCD (blood)
    • Microbiome & Metabolomics
      • All Microbiome & Metabolomics Collection kits
      • Microbiome
        • Gut
          • OMNIgene•GUT | OM-200 (feces)
          • OMNIgene•GUT | OM-200+ACP102
          • OMNIgene•GUT | DNA & RNA | OMR-205
        • Oral
          • OMNIgene•ORAL | OM-501 (saliva)
          • OMNIgene•ORAL | OM-505 (saliva)
          • OMNIgene•ORAL | OME-505 (saliva)
          • OMNIgene•ORAL | OMR-110 (gum/plaque)
          • OMNIgene•ORAL | OMR-120 (tongue)
        • Skin
          • OMNIgene•SKIN | OMR-140
        • Vaginal
          • OMNIgene•VAGINAL | OMR-130
      • Metabolomics
        • Gut
          • OMNImet•GUT | ME-200
    • Infectious Disease
      • COVID-19
        • COVID-19 Collection Kits
        • OMNIgene•ORAL | OME-505 (saliva)
        • ORAcollect•RNA | ORE-100 (saliva)
      • DNA & RNA Collection Kits
        • All DNA & RNA Collection Kits
        • OMNIgene•ORAL | OM-501 (saliva)
        • Colli-Pee | FV-5000 (urine)
      • DNA Extraction Reagents
        • prepIT•L2P | PT-L2P (saliva)
        • prepIT•Q2A | PT-Q2A (saliva)
    • Animal
      • Animal Microbiome
        • PERFORMAbiome•GUT | PB-200
      • Animal Genetics
        • PERFORMAgene | PG-100
    • Customization Options
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    • Accessories
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      Amplicon and whole genome sequencing and analysis
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      Product customization and direct-to-donor shipments
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