Oragene•DNA / North America

Description of Services

• Genome characterization and sequencing
• Genotyping assay development and validation
• Gene expression profiling and validation
• Drug efficacy and safety-related analysis
• Biologics evaluation and safety testing
• Data management, analysis and interpretation
• Sample management and biobanking
• Scientific consulting

Description of Services

DNA and RNA extractions, Realtime PCR analysis, SNP analysis using Sequenoms iPLEX system, expression analysis using the Taqman system. Manual extraction of RNA and DNA. We utilize our proprietary DNA extraction reagents, DNAQuick for DNA extraction from most of the sources. We also utilize Qiagen column protocols for some sample types. For RNA extractions we typically use Tri-reagent method and some times utilize Qiagen column based protocols. We have Standard Operating Protocols (SOPs) for all the different applications.

Description of Services

Molecular Diagnostics

• DNA mutation testing for twenty single gene disorders, Ashkenazi Jewish panel, thrombophilia, mitochondrial DNA disorders.
• Microarray-based comparative genomic hybridization for mental retardation/developmental delay (Agilent Array)
• DNA sequence analysis for disease predisposition genes.

Cytogenetics

• Chromosome and FISH analysis on amniotic fluid, peripheral blood, products of conception, fibroblasts, bone marrow, lymph node and solid tumors.

Biochemical Genetics

• Amino acid and organ acid disorders, mucopolysaccharidoses, defects of carnitine metabolism Diagnostic Microarray Services.
• Affymetrix Gene Expression, SNP analysis and Targeted Genotyping available in our clinical lab at the Center for Applied Genomics www.cag.icph.org
• Proteomic Diagnostics Clinical proteomic assays using MSIA technology which couples antibody affinity capture to mass spectrometry.

Proteomic Diagnostics

• Clinical proteomic assays using MSIA technology which couples antibody affinity capture to mass spectrometry.

Description of Services

CLIA certified services for custom molecular assays. CRL also performs clinical trials, insurance, toxicology and bioanalytical testing.

Description of Services

Expression Analysis provides expression profiling and genotyping services using Affymetrix, Illumina, Luminex and Applied Biosystems' TaqMan® technologies. As the leading provider of microarray services in clinical trials, Expression Analysis offers solutions for challenging specimens, such as blood and FFPE tissues, as well as nucleic acid isolation and data analysis services. The company also performs diagnostic assays in its CLIA and GLP-compliant laboratory.

Lab location and locations served

The Expression Analysis facility is located in Durham, NC. Our services are provided worldwide to a variety of clients including most of the top 10 pharmaceutical companies.

Expression Analysis, Inc.
4324 South Alston Avenue, Suite 101
Durham, NC 27713

Description of Services

Clinical molecular diagnostic laboratory (see website for details of services offered)

Lab location:

Molecular Genetics Laboratory
555 University Ave., Roy C. Hill Wing, Rm. 3421
Toronto, ON, Canada
M5G 1X

Description of Services

The UCLA Biomarker Innovations Laboratory (BIL) is a California state licensed and CLIA-certified clinical laboratory founded by Drs. Scott Binder and David Seligson, pathologist-scientists, and is located at one of the world's leading hospitals and academic institutions - the David Geffen School of Medicine at the University of California, Los Angeles (UCLA).

Applications:

The BIL is currently offering the J&J/Veridex CTC (Circulating Tumor Cell Detection in whole blood) assay for monitoring metastatic breast, colon and prostate cancers. EGFRvIII RT PCR Gene Expression -Glioblastoma multiforme (GBM) is the most aggressive brain tumor in adults and remains incurable despite multimodal intensive treatment regimens. EGFRvIII is a truncated extracellular mutant of the EGF receptor (EGFR) commonly found in GBMs that confers enhanced tumorigenic behavior. This assay was developed by the UCLA Neurological research team and the laboratory procedures has been validated by the BIL. The assay includes RNA extraction from 4-8 10 um thickness of tissue sections, reverse transcribe the RNA to cDNA synthesis and followed by SYBR Green Real Time PCR using Roche LightCycler 480.

Lab Location and Locations served:

UCLA Biomarker Innovations Laboratory
710 Westwood Plaza, Room 3235
Los Angeles, CA 90095

Description of Services

Genomic Analysis (Paternity Test, Papillomavirus Genotypification, detection of KRAS, JAK-2 and Factor V mutation).

Lab location and locations served:

Vitagenesis, S.A. de C.V.
Boulevard Puerta del Sol #1005
Monterrey, Nuevo León, México, 64630