Place one sponge in cheek pouch. Gently move the sponge along the gums and inner cheeks for 30 seconds to soak up as much saliva as possible.

Once sponge is saturated with saliva, insert sponge in V-notch of funnel. Wring saliva out of sponge using a twisting and pushing motion against the inner wall of the V-notch. Saliva will flow into tube.

Repeat these steps (1 to 2) USING THE SAME SPONGE until the liquid saliva (not bubbles) reaches the fill line. Check sponge for damage each time before inserting into donor's mouth. Use second sponge if first sponge shows any signs of wear or tear. Tap tube bottom against hard surface to reduce number of bubbles.

Hold the tube upright with one hand. Close the lid with the other hand (as shown) by firmly pushing the lid until you hear a loud click. The liquid in the lid will be released into the tube to mix with the saliva. Make sure the lid is closed tightly.

Hold the tube upright. Unscrew the funnel from the tube.

Use the small cap to close the tube tightly.

Shake the capped tube for 5 seconds. Discard or recycle the funnel. Discard sponges.